The chemical characteristics of lakes change according to local conditions in their watershed: soil type, vegetation type, proximity to the coast, etc. We studied 49 lakes distributed along a 300 km longitudinal transect through Tursujuq National Park, Nunavik, in August 2015 and 2016. We present the surface limnological data on pH, alkalinity, nutrients (nitrogen and phosphorus), carbon, chlorophyll a, ions and metals (Calcium, Magnesium, Sodium, Potassium, Silica, Silver, Aluminum, Antimony, Arsenic, Barium, Beryllium, Bismuth, Boron, Cadmium, Calcium, Cerium, Cesium, Chromium, Cobalt, Copper, Gallium, Germanium, Indium, Iron, Lanthanum, Lead, Lithium, Magnesium, Manganese, Molybdenum, Nickel, Niobium, Palladium, Platinum, Potassium, Rubidium, Scandium, Selenium, Sodium, Strontium, Tellurium, Thallium, Tin, Titanium, Tungsten, Uranium, Vanadium, Yttrium, Zinc, Zirconium). These data, sampled several metres from the shoreline, help us understand the current state of the lakes and their water chemistry in this protected study area.