Properties of Arctic lakes vary depending on the type of soil, major sources of water, size of the lake, elevation and climatic conditions among others. We studied 35 lakes of different sizes and positions (headwater, midstream and downstream lakes) across the Greiner Lake watershed (69°10'35,72" N, 104°55'54,87" W) on Victoria Island, Nunavut, in August 2018 and 2019. We show limnological and metabolic data for nutrients (total nitrogen and phosphorus), dissolved organic and inorganic carbon, dissolved organic carbon characterization (SUVA254, a320, S289, FDOM), water and dissolved oxygen isotopes (d2H-H2O, d18O-H2O, d18O-O2), light (Kd, irradiance), bacterial production, primary production, respiration, and net ecosystem production for this set of lakes. These variables help understand the current state of the lakes in this watershed that is of great importance for the local Ekaluktutiak Inuit community of Cambridge Bay.